Part:BBa_K2129002

Mutant EutS with Mid expression cassette

EutS with residue replaced with an Amber stop codon for incorporation of non-canonical amino acids with a custom tRNA synthetase. Includes a medium expression promoter (BBa_J23110).

This mutated EutS may form nanocompartments that break and form when transformed with a non-canonical amino acid incorporation system for Phenylalanine-4'-Azobenzene (and having access to that compound when grown). For the purposes of the CU-Boulder 2016 iGEM project, this incorporation plasmid was provided by the Peter Shultz lab and could not be submitted to the registry as such (apologies!).

! Be advised ! We do not promise that the breakage and formation is reliable: not enough data has been collected to conclusively say so!

Please also note that this part is not useful without an incorporation system for phenylalanine-4'-azobenzene. It is not meant to be used without one.

Usage and Biology

See EutS (BBa_K2129001).

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 470
  Illegal NheI site found at 493
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 126
  
• 1000
  COMPATIBLE WITH RFC[1000]